Abstract
A dose-dependent effect of naja oxiana eichwald venom S-6 and S-7 fractions on cardiac muscle contractile activity was investigated, and it was found that they have a negative inotropic effect on muscle contraction force. S-6 fraction at a concentration of 2 μg/ml was observed to reduce cardiac muscle contraction force to 8.3±3.4% and S-7 fraction to 26.1±3.4% at 5 μg/ml. The suppressive effect of these fractions was maintained in the presence of nifedipine, but to a slightly lesser extent due to the blockade of Ca2+-channels accompanied by a decrease in the level of [Ca2+]i ions in cardiomyocytes. Blockade of Na+ channels with lidocaine also led to a noticeable suppression of the negative inotropic effect for fractions S-6 to 36.3±4.2% and S-7 to 41.6±4.4% of the control, respectively. Also, it was found that the effects of S-6 and S-7 fractions on rat aorta smooth muscle contraction strength were investigated. Considering that KCl-induced contractions of the rat aorta are mainly ensured by the activation of voltage-dependent Ca2+-channels, the observed effect of fractions S6 and S7 may be associated with inhibition of Ca2+ ion transport through these channels. The influence of fractions S6 and S7 of cobra venom on the functioning of voltage- and receptor-dependent Ca2+-channels in the rat aorta and cardiac muscle was studied.
References
Braunwald E. The war against heart failure: the Lancet lecture // The Lancet. –2015. V–38. P–1838-1845.
Schwinger R.G. Pathophysiology of heart failure // Cardiovasc Diagn Ther.–2021. V.11(1). P–263-276.
Nemtsova V., Burkard T., Vischer A.S. Hypertensive Heart Disease: A Narrative Review Series—Part 2: Macrostructural and Functional Abnormalities// Journal of Clinical Medicine–2023. V–12(17). P–1-24.
Waheed H., F Moin S., I Choudhary M. Snake venom: from deadly toxins to life-saving therapeutics // Cur. Med. Chemistry – 2017. V–24. P–1874-1891.
Pérez-Peinado C., Defaus S., Andreu D. Hitchhiking with Nature: snake venom peptides to fight cancer and superbugs // Toxins – 2020. V–12. P–255.
Yuldashev E.I., Sultanaliyeva N.M., Shkinev A.V., Sadikov E.S. Markaziy Osiyo ayrim ilonlari zaharlarining og’riq qoldirish xususiyatini o‘rganish. // Инфекция, иммунитет и фармакология – 2023. № 3. C–196-203.
Пигулевский С.В. Ядовитые животные. Токсикология позвоночных. – Медицина. Ленингр. отд-ние,–1966. С–386.
Koh D. C. I., Armugam A., Jeyaseelan K. Snake venom components and their applications in biomedicine // Cell Mol Life Sci. – 2006. vol. 63 (24). p. 3030-3041
Ojeda PG, Ramírez D, Alzate-Morales J. et. Computational studies of snake venom toxins // Toxins.–2018. V–10 (8). P–1-8.
Вульфиус ЕА, Старков ВГ, Андреева ТВ, Цетлин ВИ, Уткин ЮН. Новые антагонисты никотиновых холинорецепторов - белки из ядов змей семейства Viperidae // Доклады академии наук РФ. –2015. Т– 461. № 5, C–604-607.